白色念珠菌培养物诱癌实验研究

河北省赞皇县是胃癌高发区,本研究从该地区慢性胃病患者胃液内分离出白色念珠菌並进行培养,将培养物参入饲料喂养NIH小鼠进行诱癌实验。结果诱发了小鼠肺腺癌和腺胃粘膜不典型增生,其发病率分别为26.1％(6/23)和30.4％(7/23)。对照组没有相同的病变发生。结合癌高发区现场情况,该结果提示白色念珠菌可能与肺癌和胃癌的发生有关。     

The role of pH in salivary histidine-rich polypeptide antifungal germ tube inhibitory activity

Purified synthetic salivary histidine-rich polypeptides (HRPs) 2, 3, 4, 5 and 6 were found to inhibit Candida albicans conversion of blastospores to germ tubes. HRP-4 was the best inhibitor within the pH 5 to 7 range tested and all of the HRPs were observed to lose potency as the pH was raised from 5 to 7. The pH pattern obtained with a synthetic homologous histidine peptide suggested that the protonated form of the histidine imidazole residues of the HRPs was important to the germ tube antifungal activity. Similar pH inhibition profiles of germ tube formation by parotid saliva and the HRPs were also observed.     

Intrauterine candidiasis in a twin pregnancy after myomectomy, in vitro fertilization and embryo transfer

A IVF twin pregnancy in a patient who had previously undergone a myomectomy ended in a intrauterine infection, placental abruption and fetal losses at 21 weeks. Intrauterine Candidiasis may have played a role in the pathogenesis of the unsuccesful outcome.     

Scanning Electron Microscopy of Candida albicans Chlamydospores

Summary:  Candida albicans chlamydospores were studied using scanning electron microscopy method. The various relationships of chlamydospore and pseudohyphae were documented as follow: terminal chlamydospore with or without suspensor cell; lateral chlamydospore with or without suspensor cell; terminal chlamydospore pairs with or without suspensor cell; lateral chlamydospore pairs without suspensor cell.
Zusammenfassung:  Die Chlamydosporen von Candida albicans wurden rasterelektronenmikroskopisch untersucht. Die unterschiedlichen Beziehungen zwischen Chlamydosporen und Pseudohyphen werden wie folgt beschrieben: Terminate Chlamydospore mit oder ohne Suspensorzelle; laterale Chlamydospore mit oder ohne Suspensorzelle; terminale Chlamydosporen-Paare mit oder ohne Suspensorzelle; laterale Chlamydosporen-Paare ohne Suspensorzelle.     

口腔扁平苔藓患者口腔内白色念珠菌的溶血活性研究

目的：检测白色念珠菌(C．a)是否产生溶血因子及来自正常人、非糜烂型口腔扁平苔藓(OLP)患者和糜烂型OLP患者口腔内的C．a的溶血活性是否不同。方法：用浓缩漱口水法收集口腔微生物，在SDA上培养、分离、纯化和鉴定C．a,接种于血琼脂平板上，厌氧培养，检测其溶血活性。结果：所有C．a在此条件下均产生了溶血因子，以培养48小时最明显；来自正常人、非糜烂型口腔扁平苔藓(OLP)患者和糜烂型OLP患者口腔内的C．a的溶血活性无显著性差异。结论：C．a能产生溶血因子；OLP患者有必要进行抗真菌治疗。     

Atopic asthma caused by Candida albicans acid protease: case reports

Two cases of atopic asthma caused by acid protease produced by Candida albicans are reported. Both patients had high levels of serum IgE antibodies against the acid protease and showed positive conjunctival and immediate bronchial responses when challenged with the protease. Significant histamine release was detected in both patients when their peripheral leukocytes were challenged with the protease antigen. These findings clearly showed that C. albicans acid protease is the causative allergen of atopic asthma.